Studies with electron microscopic autoradiography of thyroxine 125 I in organotypic cultures of the CNS. I. Fixation of thyroxine 125 I.
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منابع مشابه
Studies with electron microscopic autoradiography of thyroxine 125 I in organotypic cultures of the CNS. II. Sites of cellular localization of thyroxine 125 I.
Thlyroxine, important in the metamorplhosis of amphibians, as well as in the (lifferentiation of mammalian tissues, exerts its effects on suclh diverse tissues as cartilage, liver and brain(l). Effects of thyroxinie at the celltular level have been variously ascribed to its uncoupling of oxidlative phosphorylation(2), its direct interaction with enzymes(3,4), and its stimulation of mitochondria...
متن کاملThyroid hormone action in cell culture: domonstration of nuclear receptors in intact cells and isolated nuclei.
Triiodothyronine and thyroxine induce a 3-fold increase in the rate of growth of GH(1) cells in culture. To study further the action of these hormones, we examined the binding of [(125)I]triiodothyronine and purified [(125)I]thyroxine to cellular fractions after incubation with intact cells in serum-free medium. High-affinity, low-capacity binding sites for the hormones were demonstrated in nuc...
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BACKGROUND Thyroxine (T4) hormone is synthesized by the thyroid gland and then released into the systemic circulation where it binds to a number of proteins. Dysfunction in T4 transport mechanisms has been demonstrated in multiple central nervous system (CNS) diseases including Alzheimer's disease. In the presence of different compounds that inhibit potential T4 transport mechanisms, this study...
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Studies of the rate of extrathyroidal conversion of thyroxine (T4) to 3,5,3'-triiodo-L-thyronine (T3) were carried out in rats. Total body homogenates were prepared and extracted with ethanol 48, 72, and 96 hr after the intravenous injection of (125)I-T4. (131)I-T3 was added, and the paper chromatographic purification of T3 was effected by serial elution and rechromatography in three paper and ...
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ورودعنوان ژورنال:
- The Yale Journal of Biology and Medicine
دوره 45 شماره
صفحات -
تاریخ انتشار 1972